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验证数据展示

  • WB analysis using 31210-1-AP

    WB analysis using 31210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31210-1-AP (TMEM174 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293T using 31210-1-AP

    WB analysis of HEK-293T using 31210-1-AP

    WB result of TMEM174 antibody (31210-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM174 transfected HEK-293T cells.

  • IP experiment of Jurkat using 31210-1-AP

    IP experiment of Jurkat using 31210-1-AP

    IP result of anti-TMEM174 (IP:31210-1-AP, 4ug; Detection:31210-1-AP 1:800) with Jurkat cells lysate 1360 ug.

  • Featured Product
  • KD/KO Validated

TMEM174 Polyclonal antibody

TMEM174 Polyclonal Antibody for WB, IP, ELISA
Cat No. 31210-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

transmembrane protein 174

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HEK-293T cells, Jurkat cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31210-1-AP targets TMEM174 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35041

Product name: Recombinant human TMEM174 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-243 aa of BC019346

Sequence: MEQGSGRLEDFPVNVFSVTPYTPSTADIQVSDDDKAGATLLFSGIFLGLVGITFTVMGWIKYQGVSHFEWTQLLGPVLLSVGVTFILIAVCKFKMLSCQLCKESEERVPDSEQTPGGPSFVFTGINQPITFHGATVVQYIPPPYGSPEPMGINTSYLQSVVSPCGLITSGGAAAAMSSPPQYYTIYPQDNSAFVVDEGCLSFTDGGNHRPNPDVDQLEETQLEEEACACFSPPPYEEIYSLPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 174
别名 transmembrane protein 174
观测分子量 55-60 kDa
GenBank蛋白编号BC019346
基因名称 TMEM174
Gene ID (NCBI) 134288
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8WUU8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 174 (TMEM174) is comprised of 243 amino acids, and contains two predicted transmembrane helices which determine its subcellular localization in endoplasmic reticulum and influences its functions (PMID: 20331980). TMEM174) mRNA is easily detectable in human kidney tissues and activates AP-1 and promotes 293T cell proliferation (PMID: 25202393). The Tmem174 protein was extremely highly expressed in the kidney and localized at the apical membrane of the renal proximal tubules. Tmem174 binds to NaPi2a on the cell membrane and is involved in the internalization of NaPi2a (PMID: 35428804). Tmem174 is considered to be a component of the NaPi2a/NHERF1 complex that receives signals from PTH and FGF23 (PMID: 35428804). This antibody may recognize the dimer.

实验方案

Product Specific Protocols
IP protocol for TMEM174 antibody 31210-1-APDownload protocol
WB protocol for TMEM174 antibody 31210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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