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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, FC (Intra) and More (2)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19851

Print datasheet

Synonyms

STING, NET23, MPYS, MITA, ERIS

验证数据展示

  • IF Staining of HT-29 using CL488-19851

    IF Staining of HT-29 using CL488-19851

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite® Plus 488 TMEM173/STING antibody (CL488-19851) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-19851

    FC experiment of HepG2 using CL488-19851

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TMEM173/STING (CL488-19851) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHT-29 cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19851-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19851 targets TMEM173/STING in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivitymouse
Immunogen TMEM173/STING fusion protein Ag13921 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 173
Synonyms STING, NET23, MPYS, MITA, ERIS
Calculated Molecular Weight 379 aa, 42 kDa
GenBank Accession NumberBC047779
Gene Symbol STING
Gene ID (NCBI) 340061
RRIDAB_2883086
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WV6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TMEM173/STING antibody CL488-19851Download protocol
FC protocol for CL Plus 488 TMEM173/STING antibody CL488-19851Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Life Sci

Icariside II alleviates lipopolysaccharide-induced acute lung injury by inhibiting lung epithelial inflammatory and immune responses mediated by neutrophil extracellular traps

Authors - Xiuchun Li
View Article