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验证数据展示

  • WB analysis of THP-1 using 19851-1-AP

    WB analysis of THP-1 using 19851-1-AP

    WB result of TMEM173/STING antibody (19851-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells.

  • WB analysis of HepG2 using 19851-1-AP

    WB analysis of HepG2 using 19851-1-AP

    WB result of TMEM173/STING antibody (19851-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and TMEM173/STING knockout HepG2 cells.

  • WB analysis using 19851-1-AP

    WB analysis using 19851-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 19851-1-AP

    IHC staining of human tonsillitis using 19851-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STING antibody included
  • IHC staining of human tonsillitis using 19851-1-AP

    IHC staining of human tonsillitis using 19851-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STING antibody included
  • IF Staining of human tonsillitis using 19851-1-AP

    IF Staining of human tonsillitis using 19851-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse spleen using 19851-1-AP

    IP experiment of mouse spleen using 19851-1-AP

    IP result of anti-TMEM173/STING (IP:19851-1-AP, 4ug; Detection:19851-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

  • IF Staining of HT-29 using 19851-1-AP

    IF Staining of HT-29 using 19851-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of THP-1 using 19851-1-AP

    FC experiment of THP-1 using 19851-1-AP

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TMEM173/STING (19851-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of mouse brain using 19851-1-AP

    IF Staining of mouse brain using 19851-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA
Cat No. 19851-1-AP

产品说明书

引用文献(454)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA and More (3)

别名

STING, Endoplasmic reticulum interferon stimulator, ERIS, MITA, MPYS

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, mouse spleen tissue, Raji cells, THP-1 cells, HT-29 cells, mouse thymus tissue, C2C12 cells, RAW 264.7 cells, mouse testis tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue, rat thymus tissue, mouse thymus tissue, human spleen tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inHT-29 cells, human tonsillitis tissue, THP-1 cells
Positive FC (Intra) detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19851-1-AP targets TMEM173/STING in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, Blocking assay
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, monkey, bovine, hamster
免疫原

CatNo: Ag13921

Product name: Recombinant human TMEM173 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 174-379 aa of BC047779

Sequence: ELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 173
别名 STING, Endoplasmic reticulum interferon stimulator, ERIS, MITA, MPYS
计算分子量 379 aa, 42 kDa
观测分子量 35-40 kDa, 80 kDa
GenBank蛋白编号BC047779
基因名称 STING
Gene ID (NCBI) 340061
RRIDAB_10665370
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WV6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

实验方案

Product Specific Protocols
IF protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IHC protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IP protocol for TMEM173/STING antibody 19851-1-APDownload protocol
WB protocol for TMEM173/STING antibody 19851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins

Authors - Huaqing Deng
View Article
mouseIF

Nature

Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.

Authors - Ting-Ting Chu
  • KO Validated
View Article
mouseWB

Nature

eccDNAs are apoptotic products with high innate immunostimulatory activity

Authors - Yuangao Wang
  • KO Validated
View Article
humanWB

Science

The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling.

Authors - Mena Abdel-Nour
View Article
humanWB,IF

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
  • KD Validated
View Article
humanWB

Cancer Cell

Mutant p53 suppresses innate immune signaling to promote tumorigenesis.

Authors - Monisankar Ghosh
  • KD Validated
View Article
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