TMEM173/STING Recombinant antibody
TMEM173/STING Recombinant Antibody for IF/ICC,IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (1)
Applications
IF/ICC,IF-P, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
7K7
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MOLT-4 cells, Jurkat cells, K-562 cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | THP-1 cells, human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
80165-1-RR targets TMEM173/STING in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | IF/ICC,IF-P, IHC, IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | mouse |
| Immunogen | TMEM173/STING fusion protein Ag13921 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | transmembrane protein 173 |
| Synonyms | FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173 |
| Calculated Molecular Weight | 379 aa, 42 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC047779 |
| Gene Symbol | TMEM173 |
| Gene ID (NCBI) | 340061 |
| RRID | AB_2918870 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q86WV6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM173/STING antibody 80165-1-RR | Download protocol |
| IHC protocol for TMEM173/STING antibody 80165-1-RR | Download protocol |
| IF protocol for TMEM173/STING antibody 80165-1-RR | Download protocol |
| IP protocol for TMEM173/STING antibody 80165-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Roflumilast attenuates neuroinflammation post retinal ischemia/reperfusion injury by regulating microglia phenotype via the Nrf2/STING/NF-κB pathway |
