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验证数据展示

  • WB analysis using 82983-2-RR

    WB analysis using 82983-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82983-2-RR (TMEM132E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82983-2-RR

    Affinity and Kinetic Characterization of 82983-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82983-2-RR against Human TMEM132E were performed. The affinity constant is 0.254 nM.

TMEM132E Recombinant antibody

TMEM132E Recombinant Antibody for WB, ELISA
Cat No. 82983-2-RR

产品说明书

CloneNo. 230525E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

transmembrane protein 132E, 230525E12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82983-2-RR targets TMEM132E in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 TMEM132E fusion protein Ag26772 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 132E
别名 transmembrane protein 132E, 230525E12
计算分子量 116 kDa
观测分子量100-110 kDa
GenBank蛋白编号NM_001304438
基因名称 TMEM132E
Gene ID (NCBI) 124842
RRIDAB_3670733
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6IEE7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 132E (TMEM132E) is a member of the TMEM132 family which also include TMEM132A, TMEM132B, TMEM132C, TMEM132D (PMID: 29088312). The gene of TMEM132E is located within the DFNB99 locus on chromosome 17q12 and is highly expressed in the inner ear and other tissues including brain, kidney, lung, liver, spleen, heart, small intestine, colon, thymus, and stomach (PMID: 31656313). A variant of TMEM132E has been associated with autosomal-recessive nonsyndromic hearing loss DFNB99 (PMID: 25331638).

实验方案

Product Specific Protocols
WB protocol for TMEM132E antibody 82983-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols