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验证数据展示

  • WB analysis using 87837-1-RR

    WB analysis using 87837-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87837-1-RR (TMED1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 87837-1-RR

    WB analysis of PC-12 using 87837-1-RR

    PC-12 cells were subjected to SDS PAGE followed by western blot with 87837-1-RR (TMED1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR

    IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87837-1-RR (TMED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR

    IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87837-1-RR (TMED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 87837-1-RR

    IF Staining of HeLa using 87837-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMED1 antibody (87837-1-RR, Clone: 253497F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87837-1-RR

    Affinity of 87837-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87837-1-RR against Human TMED1 were performed. The affinity constant is 34.2 pM.

TMED1 Recombinant monoclonal antibody

TMED1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 87837-1-RR

产品说明书

CloneNo. 253497F4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

IL1RL1L, IL1RL1LG, Interleukin-1 receptor-like 1 ligand, p24 family protein gamma-1, p24gamma1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHGC-27 cells, PC-12 cells, BT-549 cells, HeLa cells, K-562 cells, MDA-MB-231 cells, NIH/3T3 cells
Positive IHC detected inHuman lung cancer （Adenocarcinoma）
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87837-1-RR targets TMED1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg5903

Product name: Recombinant human TMED1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-194 aa of NM_006858

Sequence: AGPPPIQDGEFTFLLPAGRKQCFYQSAPANASLETEYQVIGGAGLDVDFTLESPQGVLLVSESRKADGVHTVEPTEAGDYKLCFDNSFSTISEKLVFFELIFDSLQDDEEVEGWAEAVEPEEMLDVKMEDIKESIETMRTRLERSIQMLTLLRAFEARDRNLQEGNLERVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane emp24 protein transport domain containing 1
别名 IL1RL1L, IL1RL1LG, Interleukin-1 receptor-like 1 ligand, p24 family protein gamma-1, p24gamma1
计算分子量25 kDa
观测分子量30 kDa
GenBank蛋白编号NM_006858
基因名称 TMED1
Gene ID (NCBI) 11018
RRIDAB_3745778
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13445
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane p24 trafficking protein 1 (TMED1) belongs to the TMED protein family, also known as the p24 family, and plays a part in protein vesicular trafficking. As the critical regulator of protein trafficking along the secretory pathway, the TMED proteins influence the composition, structure, and function of the endoplasmic reticulum and the Golgi apparatus. Disruption of the secretory pathway could affect cell differentiation and survival, ultimately resulting in disease.(PMID: 38392302)

实验方案

Product Specific Protocols
IF protocol for TMED1 antibody 87837-1-RRDownload protocol
IHC protocol for TMED1 antibody 87837-1-RRDownload protocol
WB protocol for TMED1 antibody 87837-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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