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验证数据展示

  • WB analysis using 87837-1-RR (same clone as 87837-1-PBS)

    WB analysis using 87837-1-RR (same clone as 87837-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87837-1-RR (TMED1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-12 using 87837-1-RR (same clone as 87837-1-PBS)

    WB analysis of PC-12 using 87837-1-RR (same clone as 87837-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 87837-1-RR (TMED1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR (same clone as 87837-1-PBS)

    IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR (same clone as 87837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87837-1-RR (TMED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR (same clone as 87837-1-PBS)

    IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR (same clone as 87837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87837-1-RR (TMED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87837-1-RR (same clone as 87837-1-PBS)

    IF Staining of HeLa using 87837-1-RR (same clone as 87837-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMED1 antibody (87837-1-RR, Clone: 253497F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • Affinity of 87837-1-RR

    Affinity of 87837-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87837-1-RR against Human TMED1 were performed. The affinity constant is 34.2 pM.

TMED1 Recombinant monoclonal antibody, PBS Only

TMED1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87837-1-PBS

产品说明书

CloneNo. 253497F4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

IL1RL1L, IL1RL1LG, Interleukin-1 receptor-like 1 ligand, p24 family protein gamma-1, p24gamma1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87837-1-PBS targets TMED1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg5903

Product name: Recombinant human TMED1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-194 aa of NM_006858

Sequence: AGPPPIQDGEFTFLLPAGRKQCFYQSAPANASLETEYQVIGGAGLDVDFTLESPQGVLLVSESRKADGVHTVEPTEAGDYKLCFDNSFSTISEKLVFFELIFDSLQDDEEVEGWAEAVEPEEMLDVKMEDIKESIETMRTRLERSIQMLTLLRAFEARDRNLQEGNLERVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane emp24 protein transport domain containing 1
别名 IL1RL1L, IL1RL1LG, Interleukin-1 receptor-like 1 ligand, p24 family protein gamma-1, p24gamma1
计算分子量25 kDa
观测分子量30 kDa
GenBank蛋白编号NM_006858
基因名称 TMED1
Gene ID (NCBI) 11018
RRIDAB_3745778
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13445
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transmembrane p24 trafficking protein 1 (TMED1) belongs to the TMED protein family, also known as the p24 family, and plays a part in protein vesicular trafficking. As the critical regulator of protein trafficking along the secretory pathway, the TMED proteins influence the composition, structure, and function of the endoplasmic reticulum and the Golgi apparatus. Disruption of the secretory pathway could affect cell differentiation and survival, ultimately resulting in disease.(PMID: 38392302)

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