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Host
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with FITC Plus Anti-Mouse CD11b and 0.25 ug CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (CL647-98018, Clone: 230463C7) or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (CL647-98018, Clone: 230463C7) (red) or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody

TLR4/CD284 Uni-rAb® Recombinant Antibody for FC
Cat No. CL647-98018

产品说明书

CloneNo. 230463C7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Tlr4, CD284, toll like receptor 4, 230463C7

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC detected inBalb/c mouse peritoneal macrophages
For other applications, we recommend the unconjugated version of this antibody, 98018-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98018 targets TLR4/CD284 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Ag32998

Product name: Recombinant mouse Tlr4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 198-395 aa of NM_021297

Sequence: RENPQVNLSLDMSLNPIDFIQDQAFQGIKLHELTLRGNFNSSNIMKTCLQNLAGLHVHRLILGEFKDERNLEIFEPSIMEGLCDVTIDEFRLTYTNDFSDDIVKFHCLANVSAMSLAGVSIKYLEDVPKHFKWQSLSIIRCQLKQFPTLDLPFLKSLTLTMNKGSISFKKVALPSLSYLDLSRNALSFSGCCSYSDLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 toll-like receptor 4
别名 Tlr4, CD284, toll like receptor 4, 230463C7
计算分子量96 kDa
GenBank蛋白编号NM_021297
基因名称 Tlr4
Gene ID (NCBI) 21898
RRIDAB_3673659
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9QUK6
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 TLR4/CD284 antibody CL647-98018Download protocol
Standard Protocols
Click here to view our Standard Protocols
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