验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-98376-5 targets TLR2 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
DNA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | toll-like receptor 2 |
| 别名 | CD282, TIL4, toll like receptor 2, Toll/interleukin-1 receptor-like protein 4, Toll-like receptor 2 |
| 计算分子量 | 784 aa, 90 kDa |
| GenBank蛋白编号 | BC033756 |
| 基因名称 | TLR2 |
| Gene ID (NCBI) | 7097 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O60603 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 TLR2 antibody CL647-98376-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
