×
Use Able AI chat for product recommendations

验证数据展示

  • FC experiment of human PBMCs using 98376-5-RR

    FC experiment of human PBMCs using 98376-5-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR2 Rabbit RecAb (98376-5-RR, Clone: 251864G3) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98376-5-RR

    FC experiment of human PBMCs using 98376-5-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR2 Rabbit RecAb (98376-5-RR, Clone: 251864G3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity of 98376-5-RR

    Affinity of 98376-5-RR

    Biolayer interferometry (BLl) kinetic assays of 98376-5-RR against Human TLR2 were performed. The affinity constant is 89.4 nM.

Anti-Human TLR2 Rabbit Recombinant Antibody

TLR2 Uni-rAb® Recombinant Antibody for FC
Cat No. 98376-5-RR

产品说明书

CloneNo. 251864G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD282, TIL4, toll like receptor 2, Toll/interleukin-1 receptor-like protein 4, Toll-like receptor 2

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98376-5-RR targets TLR2 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

DNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 toll-like receptor 2
别名 CD282, TIL4, toll like receptor 2, Toll/interleukin-1 receptor-like protein 4, Toll-like receptor 2
计算分子量 784 aa, 90 kDa
GenBank蛋白编号BC033756
基因名称 TLR2
Gene ID (NCBI) 7097
RRIDAB_3746022
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60603
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).

实验方案

Product Specific Protocols
FC protocol for TLR2 antibody 98376-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...