- Featured Product
- KD/KO Validated
CoraLite® Plus 750-conjugated TLE1 Monoclonal antibody
TLE1 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
2F2H11
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, NIH/3T3 cells |
For other applications, we recommend the unconjugated version of this antibody, 66216-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-66216 targets TLE1 in WB applications and shows reactivity with human samples.
| Tested Applications | WB |
| Tested Reactivity | human |
| Immunogen | TLE1 fusion protein Ag21327 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) |
| Synonyms | E(Sp1) homolog, ESG, ESG1, GRG1, TLE1 |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 85-95 kDa |
| GenBank Accession Number | BC010100 |
| Gene Symbol | TLE1 |
| Gene ID (NCBI) | 7088 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q04724 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transducin-like enhancer protein 1(TLE1) is a transcriptional corepressor. It can bind to and regress expression of many transcription factors such as NFkB, which is mediated by FOXA2, CTNNB1 and TCF family member in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Expression of TLE1 is highest in skeletal muscle and liver, moderate in placenta, heart, kidney, and spleen, and weak in all other tissues including brain.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 TLE1 antibody CL750-66216 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |