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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TIPE2 Polyclonal antibody

TIPE2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-15940

Print datasheet

Synonyms

FLJ23467, Inflammation factor protein 20, TIPE2, TNFAIP8 like protein 2, TNFAIP8L2

验证数据展示

  • FC experiment of THP-1 using CL488-15940

    FC experiment of THP-1 using CL488-15940

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TIPE2 (CL488-15940) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inTHP-1 cells
For other applications, we recommend the unconjugated version of this antibody, 15940-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15940 targets TIPE2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen TIPE2 fusion protein Ag7964 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor necrosis factor, alpha-induced protein 8-like 2
Synonyms FLJ23467, Inflammation factor protein 20, TIPE2, TNFAIP8 like protein 2, TNFAIP8L2
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC063014
Gene Symbol TNFAIP8L2
Gene ID (NCBI) 79626
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P589
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role for a signal transduction pathway that links inflammatory immune response to specific conditions after cerebral ischemia

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TIPE2 antibody CL488-15940Download protocol
Standard Protocols
Click here to view our Standard Protocols