验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, mouse small intestine tissue, mouse cerebellum tissue, rat lymph tissue, rat spleen tissue, RAW 264.7 cells, mouse spleen tissue |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human lymphoma tissue, human ovary tumor tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | RAW 264.7 cells, HepG2 cells |
| Positive FC (Intra) detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15940-1-AP targets TNFAIP8L2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | TNFAIP8L2 fusion protein Ag7964 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor, alpha-induced protein 8-like 2 |
| 别名 | TIPE2, Inflammation factor protein 20, Tumor necrosis factor alpha-induced protein 8-like protein 2, TNFAIP8-like protein 2, TNFAIP8 like protein 2 |
| 计算分子量 | 184 aa, 21 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC063014 |
| 基因名称 | TIPE2 |
| Gene ID (NCBI) | 79626 |
| RRID | AB_2204663 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P589 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role for a signal transduction pathway that links inflammatory immune response to specific conditions after cerebral ischemia
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TNFAIP8L2 antibody 15940-1-AP | Download protocol |
| IHC protocol for TNFAIP8L2 antibody 15940-1-AP | Download protocol |
| IF protocol for TNFAIP8L2 antibody 15940-1-AP | Download protocol |
| IP protocol for TNFAIP8L2 antibody 15940-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. | ||
Nat Immunol Direction of leukocyte polarization and migration by the phosphoinositide-transfer protein TIPE2.
| ||
Sci Adv TIPE2 is a checkpoint of natural killer cell maturation and antitumor immunity.
| ||
Autophagy TNFAIP8L2/TIPE2 impairs autolysosome reformation via modulating the RAC1-MTORC1 axis.
| ||
J Exp Med TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.
| ||
Cancer Immunol Res Tumor Necrosis Factor-α-Induced Protein 8-Like 2 Fosters Tumor-Associated Microbiota to Promote the Development of Colorectal Cancer. |
