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验证数据展示

  • IF Staining of mouse spleen using CL532-83545

    IF Staining of mouse spleen using CL532-83545

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite®532 TIGIT antibody (CL532-83545, Clone: 240423D3 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite®532-conjugated TIGIT Recombinant antibody

TIGIT Recombinant Antibody for IF-P
Cat No. CL532-83545

产品说明书

CloneNo. 240423D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®532
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse spleen tissue
For other applications, we recommend the unconjugated version of this antibody, 83545-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL532-83545 targets TIGIT in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 T cell immunoreceptor with Ig and ITIM domains
别名 VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains
计算分子量26 kDa
观测分子量44 kDa
GenBank蛋白编号NM_173799.4
基因名称 TIGIT
Gene ID (NCBI) 201633
RRIDAB_3673402
偶联类型 CoraLite®532 Fluorescent Dye
最大激发/发射波长537 nm / 560 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ495A1-1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).

实验方案

Product Specific Protocols
IF protocol for CL532 TIGIT antibody CL532-83545Download protocol
Standard Protocols
Click here to view our Standard Protocols