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验证数据展示

  • IF Staining of PC-3 using 84064-6-RR

    IF Staining of PC-3 using 84064-6-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using TICAM2 antibody (84064-6-RR, Clone: 241186C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse testis using 84064-6-RR

    IHC staining of mouse testis using 84064-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84064-6-RR (TICAM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TICAM2 Recombinant antibody

TICAM2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84064-6-RR

产品说明书

CloneNo. 241186C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

241186C12, MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84064-6-RR targets TICAM2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 toll-like receptor adaptor molecule 2
别名 241186C12, MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2
计算分子量 27 kDa
GenBank蛋白编号NM_021649
基因名称 TICAM2
Gene ID (NCBI) 353376
RRIDAB_3671634
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ86XR7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TICAM2, also named TIRAP3, TIRP, and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.

实验方案

Product Specific Protocols
IHC protocol for TICAM2 antibody 84064-6-RRDownload protocol
IF protocol for TICAM2 antibody 84064-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols