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验证数据展示

  • WB analysis using 85093-2-RR

    WB analysis using 85093-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85093-2-RR (THRAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 85093-2-RR

    WB analysis of human brain using 85093-2-RR

    human brain tissue were subjected to SDS PAGE followed by western blot with 85093-2-RR (THRAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85093-2-RR

    IF Staining of HeLa using 85093-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THRAP3 antibody (85093-2-RR, Clone: 242268D8 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

THRAP3 Recombinant antibody

THRAP3 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85093-2-RR

产品说明书

CloneNo. 242268D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

TRAP150, Thyroid hormone receptor-associated protein complex 150 kDa component, Thyroid hormone receptor-associated protein 3, BCLAF2, BCLAF1 and THRAP3 family member 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, U-251 cells, LNCaP cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy THRAP3 Ready-To-Use IHC Kit. THRAP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85093-2-RR targets THRAP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 thyroid hormone receptor associated protein 3
别名 TRAP150, Thyroid hormone receptor-associated protein complex 150 kDa component, Thyroid hormone receptor-associated protein 3, BCLAF2, BCLAF1 and THRAP3 family member 2
计算分子量 109 kDa
观测分子量109-130 kDa
GenBank蛋白编号NM_005119
基因名称 THRAP3
Gene ID (NCBI) 9967
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y2W1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THRAP3, also named as TRAP150, plays a role in transcriptional coactivation.

实验方案

Product Specific Protocols
WB protocol for THRAP3 antibody 85093-2-RRDownload protocol
IF protocol for THRAP3 antibody 85093-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols