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验证数据展示

  • IF Staining of HeLa using CL488-66114

    IF Staining of HeLa using CL488-66114

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TFIP11 antibody (CL488-66114, Clone: 1A1C8 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated TFIP11 Monoclonal antibody

TFIP11 Monoclonal Antibody for IF/ICC
Cat No. CL488-66114

产品说明书

CloneNo. 1A1C8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC

别名

bK445C9.6, FLJ22086, hNtr1, NTR1, STIP, STIP 1, TFIP11, TIP39

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66114-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66114 targets TFIP11 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag5821

Product name: Recombinant human TFIP11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 492-837 aa of BC033080

Sequence: IVTQWQPRNCDPMVDFLDSWVHIIPVWILDNILDQLIFPKLQKEVENWNPLTDTVPIHSWIHPWLPLMQARLEPLYSPIRSKLSSALQKWHPSDSSAKLILQPWKDVFTPGSWEAFMVKNIVPKLGMCLGELVINPHQQHMDAFYWVIDWEGMISVSSLVGLLEKHFFPKWLQVLCSWLSNSPNYEEITKWYLGWKSMFSDQVLAHPSVKDKFNEALDIMNRAVSSNVGAYMQPGARENIAYLTHTERRKDFQYEAMQERREAENMAQRGIGVAASSVPMNFKDLIETKAEEHNIVFMPVIGKRHEGKQLYTFGRIVIYIDRGVVFVQGEKTWVPTSLQSLIDMAK

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tuftelin interacting protein 11
别名 bK445C9.6, FLJ22086, hNtr1, NTR1, STIP, STIP 1, TFIP11, TIP39
计算分子量 97 kDa
观测分子量 97 kDa
GenBank蛋白编号BC033080
基因名称 TFIP11
Gene ID (NCBI) 24144
RRIDAB_2883261
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBB9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TFIP11 antibody CL488-66114Download protocol
Standard Protocols
Click here to view our Standard Protocols