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TFIP11 Monoclonal antibody

TFIP11 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A1C8

Cat No : 66114-1-Ig

Print datasheet

Synonyms

bK445C9.6, STIP, STIP 1, TFIP11, TIP39

验证数据展示

  • WB analysis using 66114-1-Ig

    WB analysis using 66114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66114-1-Ig

    IHC staining of human breast cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66114-1-Ig

    IF Staining of HeLa using 66114-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66114-1-Ig(TFIP11 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66114-1-Ig

    WB analysis using 66114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • WB analysis using 66114-1-Ig

    WB analysis using 66114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66114-1-Ig

    IF Staining of HeLa using 66114-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody (66114-1-Ig, Clone: 1A1C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, human testis tissue, human brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, K-562 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66114-1-Ig targets TFIP11 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen TFIP11 fusion protein Ag5821 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tuftelin interacting protein 11
Synonyms bK445C9.6, STIP, STIP 1, TFIP11, TIP39
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC033080
Gene Symbol TFIP11
Gene ID (NCBI) 24144
RRIDAB_2881513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBB9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.

实验方案

Product Specific Protocols
WB protocol for TFIP11 antibody 66114-1-IgDownload protocol
IHC protocol for TFIP11 antibody 66114-1-IgDownload protocol
IF protocol for TFIP11 antibody 66114-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols