验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-14572 targets TFAP2C/AP2 gamma in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag5648 Product name: Recombinant human TFAP2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-450 aa of BC035664 Sequence: MLWKITDNVKYEEDCEDRHDGSSNGNPRVPHLSSAGQHLYSPAPPLSHTGVAEYQPPPYFPPPYQQLAYSQSADPYSHLGEAYAAAINPLHQPAPTGSQQQAWPGRQSQEGAGLPSHHGRPAGLLPHLSGLEAGAVSARRDAYRRSDLLLPHAHALDAAGLAENLGLHDMPHQMDEVQNVDDQHLLLHDQTVIRKGPISMTKNPLNLPCQKELVGAVMNPTEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAAHVTLLTSLVEGEAVHLARDFAYVCEAEFPSKPVAEYLTRPHLGGRNEMAARKNMLLAAQQLCKEFTELLSQDRTPHGTSRLAPVLETNIQNCLSHFSLITHGFGSQAICAAVSALQNYIKEALIVIDKSYMNPGDQSPADSNKTLEKMEKHRK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) |
| 别名 | TFAP2C, Activating enhancer-binding protein 2 gamma, ap 2gamma, AP2 GAMMA, AP2-gamma |
| 计算分子量 | 49 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC035664 |
| 基因名称 | TFAP2C |
| Gene ID (NCBI) | 7022 |
| RRID | AB_2919618 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570 nm |
| 激发激光 | Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92754 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TFAP2C (Transcription Factor AP-2 Gamma) is a member of the AP-2 transcription factor family, which plays a crucial role in early embryonic development, specifically in regulating the development of trophoblast cells and maintaining pluripotency. TFAP2C is known to regulate transcription by opening enhancers and is associated with a set of enhancers present in human embryos. It also functions as a regulator of both inner cell mass and trophectoderm differentiation, and controls the expression of cell-polarity genes. This protein binds to specific DNA sequences to activate or repress target genes, making it a key player in cellular processes and a potential therapeutic target in oncology research.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL555 TFAP2C/AP2 gamma antibody CL555-14572 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
