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- KD/KO Validated
TFAP2C Polyclonal antibody
TFAP2C Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human placenta tissue, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy TFAP2C Ready-To-Use IHC Kit. TFAP2C primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14572-1-AP targets TFAP2C in WB, IP, FC, IHC, chIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IHC, chIP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | TFAP2C fusion protein Ag5648 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) |
| Synonyms | ap 2gamma, AP2 GAMMA, ERF1, hAP 2g, TFAP2C, TFAP2G, Transcription factor ERF 1 |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC035664 |
| Gene Symbol | TFAP2C |
| Gene ID (NCBI) | 7022 |
| RRID | AB_2271424 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92754 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TFAP2C antibody 14572-1-AP | Download protocol |
| IHC protocol for TFAP2C antibody 14572-1-AP | Download protocol |
| IP protocol for TFAP2C antibody 14572-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.
| ||
Oncogene Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling. | ||
Front Oncol TFAP2C-Mediated lncRNA PCAT1 Inhibits Ferroptosis in Docetaxel-Resistant Prostate Cancer Through c-Myc/miR-25-3p/SLC7A11 Signaling. | ||
Cancers (Basel) TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB
| ||
Nat Commun Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment |
