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TFAP2C Polyclonal antibody

TFAP2C Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14572-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA and More (1)

Activating enhancer-binding protein 2 gamma, ap 2gamma, AP2 GAMMA, AP2-gamma, ERF1

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TFAP2C Ready-To-Use IHC Kit. TFAP2C primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14572-1-AP targets TFAP2C in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, chIP
经测试反应性 human
文献引用反应性human
免疫原 TFAP2C fusion protein Ag5648 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
别名 Activating enhancer-binding protein 2 gamma, ap 2gamma, AP2 GAMMA, AP2-gamma, ERF1
计算分子量 49 kDa
观测分子量 50 kDa
GenBank蛋白编号BC035664
基因名称 TFAP2C
Gene ID (NCBI) 7022
RRIDAB_2271424
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92754
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TFAP2C antibody 14572-1-APDownload protocol
IHC protocol for TFAP2C antibody 14572-1-APDownload protocol
IP protocol for TFAP2C antibody 14572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,ChIP

Mol Cancer

CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.

Authors - Tao Jiang
  • KD Validated
humanWB

Oncogene

Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

Authors - Qingcai Meng
humanchIP

Oncogene

TFAP2C regulates carbonic anhydrase XII in human breast cancer.

Authors - Christopher M Franke
humanIHC

Genes Immun

LncRNA sequencing reveals an essential role for the lncRNA-mediated ceRNA network in penile squamous cell carcinoma

Authors - Jian Cao
humanWB

Front Oncol

TFAP2C-Mediated lncRNA PCAT1 Inhibits Ferroptosis in Docetaxel-Resistant Prostate Cancer Through c-Myc/miR-25-3p/SLC7A11 Signaling.

Authors - Xingkang Jiang
humanWB,IHC

Cancers (Basel)

TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB

Authors - Ji Xing
  • KD Validated
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