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验证数据展示

  • WB analysis using 86365-1-RR (same clone as 86365-1-PBS)

    WB analysis using 86365-1-RR (same clone as 86365-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86365-1-RR (TFAP2C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86365-1-PBS in a different storage buffer formulation.

  • WB analysis using 86365-1-RR (same clone as 86365-1-PBS)

    WB analysis using 86365-1-RR (same clone as 86365-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86365-1-RR (TFAP2C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86365-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 86365-1-RR (same clone as 86365-1-PBS)

    IF Staining of MCF-7 using 86365-1-RR (same clone as 86365-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TFAP2C antibody (86365-1-RR, Clone: 250904E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86365-1-PBS in a different storage buffer formulation.

TFAP2C/AP2 gamma Recombinant monoclonal antibody, PBS Only

TFAP2C/AP2 gamma Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86365-1-PBS

产品说明书

CloneNo. 250904E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

TFAP2C, Activating enhancer-binding protein 2 gamma, ap 2gamma, AP2 GAMMA, AP2-gamma

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TFAP2C Ready-To-Use IHC Kit. TFAP2C primary antibody included.

产品信息

86365-1-PBS targets TFAP2C/AP2 gamma in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5648

Product name: Recombinant human TFAP2C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-450 aa of BC035664

Sequence: MLWKITDNVKYEEDCEDRHDGSSNGNPRVPHLSSAGQHLYSPAPPLSHTGVAEYQPPPYFPPPYQQLAYSQSADPYSHLGEAYAAAINPLHQPAPTGSQQQAWPGRQSQEGAGLPSHHGRPAGLLPHLSGLEAGAVSARRDAYRRSDLLLPHAHALDAAGLAENLGLHDMPHQMDEVQNVDDQHLLLHDQTVIRKGPISMTKNPLNLPCQKELVGAVMNPTEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAAHVTLLTSLVEGEAVHLARDFAYVCEAEFPSKPVAEYLTRPHLGGRNEMAARKNMLLAAQQLCKEFTELLSQDRTPHGTSRLAPVLETNIQNCLSHFSLITHGFGSQAICAAVSALQNYIKEALIVIDKSYMNPGDQSPADSNKTLEKMEKHRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
别名 TFAP2C, Activating enhancer-binding protein 2 gamma, ap 2gamma, AP2 GAMMA, AP2-gamma
计算分子量 49 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC035664
基因名称 TFAP2C
Gene ID (NCBI) 7022
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92754
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TFAP2C (Transcription Factor AP-2 Gamma) is a member of the AP-2 transcription factor family, which plays a crucial role in early embryonic development, specifically in regulating the development of trophoblast cells and maintaining pluripotency. TFAP2C is known to regulate transcription by opening enhancers and is associated with a set of enhancers present in human embryos. It also functions as a regulator of both inner cell mass and trophectoderm differentiation, and controls the expression of cell-polarity genes. This protein binds to specific DNA sequences to activate or repress target genes, making it a key player in cellular processes and a potential therapeutic target in oncology research.

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