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验证数据展示

  • WB analysis using 84946-5-RR (same clone as 84946-5-PBS)

    WB analysis using 84946-5-RR (same clone as 84946-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84946-5-RR (TEX264 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84946-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84946-5-RR (same clone as 84946-5-PBS)

    IHC staining of mouse testis using 84946-5-RR (same clone as 84946-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84946-5-RR (TEX264 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84946-5-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 84946-5-RR (same clone as 84946-5-PBS)

    IF Staining of Neuro-2a using 84946-5-RR (same clone as 84946-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TEX264 antibody (84946-5-RR, Clone: 242500B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84946-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84946-5-RR

    Affinity and Kinetic Characterization of 84946-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84946-5-RR against Human TEX264 were performed. The affinity constant is 0.254 nM.

TEX264 Recombinant monoclonal antibody, PBS Only

TEX264 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84946-5-PBS

产品说明书

CloneNo. 242500B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

242500B3, SIG11, TEG 264, Testis-expressed protein 264, TEX 264

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84946-5-PBS targets TEX264 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag23027

Product name: Recombinant human TEX264 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 34-313 aa of BC008742

Sequence: VEVSAGSPPIRNVTVAYKFHMGLYGETGRLFTESCSISPKLRSIAVYYDNPHMVPPDKCRCAVGSILSEGEESPSPELIDLYQKFGFKVFSFPAPSHVVTATFPYTTILSIWLATRRVHPALDTYIKERKLCAYPRLEIYQEDQIHFMCPLARQGDFYVPEMKETEWKWRGLVEAIDTQVDGTGADTMSDTSSVSLEVSPGSRETSAATLSPGASSRGWDDGDTRSEHSYSESGASGSSFEELDLEGEGPLGESRLDPGTEPLGTTKWLWEPTAPEKGKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 testis expressed 264
别名 242500B3, SIG11, TEG 264, Testis-expressed protein 264, TEX 264
计算分子量 313 aa, 34 kDa
观测分子量37-40 kDa
GenBank蛋白编号BC008742
基因名称 TEX264
Gene ID (NCBI) 51368
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y6I9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

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