• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of MCF-7 using CL555-66845

    FC experiment of MCF-7 using CL555-66845

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human TDRKH (CL555-66845, Clone:2B1B12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of U2OS using CL555-66845

    IF Staining of U2OS using CL555-66845

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite®555 TDRKH antibody (CL555-66845, Clone: 2B1B12 ) at dilution of 1:200.

CoraLite®555-conjugated TDRKH Monoclonal antibody

TDRKH Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-66845

产品说明书

CloneNo. 2B1B12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

TDRKH specific, TDRD2, tudor and KH domain containing, Tudor and KH domain-containing protein, Tudor domain-containing protein 2

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®555
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66845-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66845 targets TDRKH in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag4720

Product name: Recombinant human TDRKH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-374 aa of BC032690

Sequence: MSTERTSWTSLSTIQKIALGFGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGRQGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVSEQLSVPQRSVGRIIGRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPPKAGGDMAVVVSKEGSWEKPSDDSFQKSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIVGSRSLQLDKLVNEMTQHYENSVPEDLTVHVGDIVAAPLPTNGSWYRAR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tudor and KH domain containing
别名 TDRKH specific, TDRD2, tudor and KH domain containing, Tudor and KH domain-containing protein, Tudor domain-containing protein 2
计算分子量 606 aa, 67 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC032690
基因名称 TDRKH
Gene ID (NCBI) 11022
RRIDAB_2934679
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y2W6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

实验方案

Product Specific Protocols
FC protocol for CL555 TDRKH antibody CL555-66845Download protocol
IF protocol for CL555 TDRKH antibody CL555-66845Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...