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验证数据展示

  • WB analysis using 66845-1-Ig

    WB analysis using 66845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of HepG2 using 66845-1-Ig

    IF Staining of HepG2 using 66845-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66845-1-Ig

    WB analysis using 66845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66845-1-Ig

    WB analysis using 66845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 66845-1-Ig

    IHC staining of mouse testis using 66845-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 66845-1-Ig

    IHC staining of mouse testis using 66845-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TDRKH Monoclonal antibody

TDRKH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66845-1-Ig

产品说明书

CloneNo. 2B1B12

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

TDRD2, TDRKH, tudor and KH domain containing

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, JAR cells, NCCIT cells, LNCaP cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat testis tissue, mouse testis tissue, Rat brain
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66845-1-Ig targets TDRKH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原

CatNo: Ag4720

Product name: Recombinant human TDRKH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-374 aa of BC032690

Sequence: MSTERTSWTSLSTIQKIALGFGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGRQGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVSEQLSVPQRSVGRIIGRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPPKAGGDMAVVVSKEGSWEKPSDDSFQKSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIVGSRSLQLDKLVNEMTQHYENSVPEDLTVHVGDIVAAPLPTNGSWYRAR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tudor and KH domain containing
别名 TDRD2, TDRKH, tudor and KH domain containing
计算分子量 606 aa, 67 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC032690
基因名称 TDRKH
Gene ID (NCBI) 11022
RRIDAB_2882185
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y2W6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

实验方案

Product Specific Protocols
IF protocol for TDRKH antibody 66845-1-IgDownload protocol
IHC protocol for TDRKH antibody 66845-1-IgDownload protocol
WB protocol for TDRKH antibody 66845-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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