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验证数据展示

  • WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66845-1-Ig (same clone as 66845-1-PBS)

    IF Staining of HepG2 using 66845-1-Ig (same clone as 66845-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51542-2

    Cytometric bead array sample test of MP51542-2

    Cytometric bead array sample test of MP51542-2, TDRKH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66845-1-PBS. Detection antibody: 66845-4-PBS.

  • WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51542-2

    Cytometric bead array standard curve of MP51542-2

    Cytometric bead array standard curve of MP51542-2, TDRKH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66845-1-PBS. Detection antibody: 66845-4-PBS. Standard:Ag4720. Range: 3.125-400 ng/mL.

TDRKH Monoclonal antibody, PBS Only (Capture)

TDRKH Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66845-1-PBS

产品说明书

CloneNo. 2B1B12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

TDRKH specific, TDRD2, tudor and KH domain containing, Tudor and KH domain-containing protein, Tudor domain-containing protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66845-1-PBS targets TDRKH as part of a matched antibody pair:

MP51542-2: 66845-1-PBS capture and 66845-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag4720

Product name: Recombinant human TDRKH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-374 aa of BC032690

Sequence: MSTERTSWTSLSTIQKIALGFGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGRQGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVSEQLSVPQRSVGRIIGRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPPKAGGDMAVVVSKEGSWEKPSDDSFQKSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIVGSRSLQLDKLVNEMTQHYENSVPEDLTVHVGDIVAAPLPTNGSWYRAR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tudor and KH domain containing
别名 TDRKH specific, TDRD2, tudor and KH domain containing, Tudor and KH domain-containing protein, Tudor domain-containing protein 2
计算分子量 606 aa, 67 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC032690
基因名称 TDRKH
Gene ID (NCBI) 11022
RRIDAB_2882185
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y2W6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

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