验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MDCK cells, U251 cells |
| Positive IF/ICC detected in | U-251 cells |
| Positive FC (Intra) detected in | A431 cells, SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83227-1-RR targets TCP11L2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, canine samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, canine |
| 免疫原 |
CatNo: Ag11185 Product name: Recombinant human TCP11L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 169-519 aa of BC033617 Sequence: EHSAVDIQGLANYVISTMGKLCAPVRDNDIRELKATGNIVEVLRQIFHVLDLMQMDMANFTIMSLRPHLQRQLVEYERTKFQEILEETPSALDQTTEWIKESVNEELFSLSESALTPGAENTSKPSLSPTLVLNNSYLKLLQWDYQKKELPETLMTDGARLQELTEKLNQLKIIACLSLITNNMVGAITGGLPELASRLTRISAVLLEGMNKETFNLKEVLNSIGIQTCVEVNKTLMERGLPTLNAEIQANLIGQFSSIEEEDNPIWSLIDKRIKLYMRRLLCLPSPQKCMPPMPGGLAVIQQELEALGSQYANIVNLNKQVYGPFYANILRKLLFNEEAMGKVDASPPTN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | t-complex 11 (mouse)-like 2 |
| 别名 | T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6 |
| 计算分子量 | 519 aa, 58 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC033617 |
| 基因名称 | TCP11L2 |
| Gene ID (NCBI) | 255394 |
| RRID | AB_3670910 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N4U5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TCP11L2 is a protein containing a serine-rich region in its N-terminal region. It is a member of the TCP11 family, which includes SOK1, TCP11L1, and TCP11L2. TCP11L2 has a role in the migration and differentiation of skeletal muscle-derived satellite cells. It has been shown that Tcp11l2 expression gradually increased during the differentiation of bovine muscle-derived satellite cells. (PMID: 32017087)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for TCP11L2 antibody 83227-1-RR | Download protocol |
| IF protocol for TCP11L2 antibody 83227-1-RR | Download protocol |
| WB protocol for TCP11L2 antibody 83227-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
