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验证数据展示

  • WB analysis using 83227-1-RR (same clone as 83227-1-PBS)

    WB analysis using 83227-1-RR (same clone as 83227-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83227-1-RR (same clone as 83227-1-PBS)

    FC experiment of A431 using 83227-1-RR (same clone as 83227-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 83227-1-RR (same clone as 83227-1-PBS)

    IF Staining of U-251 using 83227-1-RR (same clone as 83227-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TCP11L2 antibody (83227-1-RR, Clone: 240096F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00177-1

    Cytometric bead array standard curve of MP00177-1

    Cytometric bead array standard curve of MP00177-1, TCP11L2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83227-1-PBS. Detection antibody: 83227-2-PBS. Standard: Ag11185. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83227-1-RR

    Affinity and Kinetic Characterization of 83227-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human TCP11L2 were performed. The affinity constant is below 1 pM.

TCP11L2 Recombinant monoclonal antibody, PBS Only (Capture)

TCP11L2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83227-1-PBS

产品说明书

CloneNo. 240096F6

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83227-1-PBS targets TCP11L2 as part of a matched antibody pair:

MP00177-1: 83227-1-PBS capture and 83227-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, canine
免疫原

CatNo: Ag11185

Product name: Recombinant human TCP11L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 169-519 aa of BC033617

Sequence: EHSAVDIQGLANYVISTMGKLCAPVRDNDIRELKATGNIVEVLRQIFHVLDLMQMDMANFTIMSLRPHLQRQLVEYERTKFQEILEETPSALDQTTEWIKESVNEELFSLSESALTPGAENTSKPSLSPTLVLNNSYLKLLQWDYQKKELPETLMTDGARLQELTEKLNQLKIIACLSLITNNMVGAITGGLPELASRLTRISAVLLEGMNKETFNLKEVLNSIGIQTCVEVNKTLMERGLPTLNAEIQANLIGQFSSIEEEDNPIWSLIDKRIKLYMRRLLCLPSPQKCMPPMPGGLAVIQQELEALGSQYANIVNLNKQVYGPFYANILRKLLFNEEAMGKVDASPPTN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 t-complex 11 (mouse)-like 2
别名 T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6
计算分子量 519 aa, 58 kDa
观测分子量60 kDa
GenBank蛋白编号BC033617
基因名称 TCP11L2
Gene ID (NCBI) 255394
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8N4U5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TCP11L2 is a protein containing a serine-rich region in its N-terminal region. It is a member of the TCP11 family, which includes SOK1, TCP11L1, and TCP11L2. TCP11L2 has a role in the migration and differentiation of skeletal muscle-derived satellite cells. It has been shown that Tcp11l2 expression gradually increased during the differentiation of bovine muscle-derived satellite cells. (PMID: 32017087)

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