验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, NCI-H1299 cells, PC-3 cells, Jurkat cells, K-562 cells, THP-1 cells, A549 cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
67140-1-Ig targets TCF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag15734 Product name: Recombinant human TCF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 318-480 aa of BC110580 Sequence: WPRAGAPGALSPSYDGGLHGLQSKIEDHLDEAIHVLRSHAVGTAGDMHTLLPGHGALASGFTGPMSLGGRHAGLVGGSHPEDGLAGSTSLMHNHAALPSQPGTLPDLSRPPDSYSGLGRAGATAAASEIKREEKEDEENTSAADHSEEEKKELKAPRARTRCQ 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) |
| 别名 | bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1 |
| 计算分子量 | 654 aa, 68 kDa |
| 观测分子量 | 68-70 kDa |
| GenBank蛋白编号 | BC110580 |
| 基因名称 | TCF3 |
| Gene ID (NCBI) | 6929 |
| RRID | AB_2882439 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15923 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TCF3 antibody 67140-1-Ig | Download protocol |
| WB protocol for TCF3 antibody 67140-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Reproduction TCF3 regulates human endometrial stromal cell proliferation and migration in RPL.
| ||
Mol Med Rep Expression of TCF3 in Wilms' tumor and its regulatory role in kidney tumor cell viability, migration and apoptosis in vitro.
| ||
Cancer Med Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling |
