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验证数据展示

  • WB analysis using 67140-1-Ig (same clone as 67140-1-PBS)

    WB analysis using 67140-1-Ig (same clone as 67140-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67140-1-Ig (same clone as 67140-1-PBS)

    IF Staining of HepG2 using 67140-1-Ig (same clone as 67140-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TCF3 antibody (67140-1-Ig, Clone: 1D12E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67140-1-Ig (same clone as 67140-1-PBS)

    IF Staining of HepG2 using 67140-1-Ig (same clone as 67140-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67140-1-Ig (TCF3 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

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  • KD/KO验证

TCF3 Monoclonal antibody, PBS Only

TCF3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67140-1-PBS

产品说明书

CloneNo. 1D12E5

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, Indirect ELISA

别名

bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67140-1-PBS targets TCF3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag15734

Product name: Recombinant human TCF3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 318-480 aa of BC110580

Sequence: WPRAGAPGALSPSYDGGLHGLQSKIEDHLDEAIHVLRSHAVGTAGDMHTLLPGHGALASGFTGPMSLGGRHAGLVGGSHPEDGLAGSTSLMHNHAALPSQPGTLPDLSRPPDSYSGLGRAGATAAASEIKREEKEDEENTSAADHSEEEKKELKAPRARTRCQ

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
别名 bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1
计算分子量 654 aa, 68 kDa
观测分子量 68-70 kDa
GenBank蛋白编号BC110580
基因名称 TCF3
Gene ID (NCBI) 6929
RRIDAB_2882439
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15923
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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