验证数据展示
经过测试的应用
| Positive WB detected in | H9C2 cells, mouse heart tissue, rat heart tisue |
| Positive FC (Intra) detected in | k562 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83414-5-RR targets TBX20 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18501 Product name: Recombinant human TBX20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-119 aa of BC120946 Sequence: MEFTASPKPQLSSRANAFSIAALMSSGGSKEKEATENTIKPLEQFVEKSSCAQPLGELTSLDAHGEFGGGSGSSPSSSSLCTEPLIPTTPIIPSEEMAKIACSLETKELWDKFHELGTE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | T-box 20 |
| 别名 | TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4 |
| 计算分子量 | 297 aa, 33 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC120946 |
| 基因名称 | TBX20 |
| Gene ID (NCBI) | 57057 |
| RRID | AB_3671063 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9UMR3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBX20 antibody 83414-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
