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验证数据展示

  • WB analysis using 83414-5-RR (same clone as 83414-5-PBS)

    WB analysis using 83414-5-RR (same clone as 83414-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83414-5-RR (TBX20 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83414-5-PBS in a different storage buffer formulation.

  • FC experiment of k562 using 83414-5-RR (same clone as 83414-5-PBS)

    FC experiment of k562 using 83414-5-RR (same clone as 83414-5-PBS)

    1x10^6 k562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-5-RR, Clone:240385C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83414-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83414-5-RR

    Affinity and Kinetic Characterization of 83414-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83414-5-RR against Human TBX20 were performed. The affinity constant is 30 nM.

TBX20 Recombinant monoclonal antibody, PBS Only

TBX20 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83414-5-PBS

产品说明书

CloneNo. 240385C12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83414-5-PBS targets TBX20 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18501

Product name: Recombinant human TBX20 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-119 aa of BC120946

Sequence: MEFTASPKPQLSSRANAFSIAALMSSGGSKEKEATENTIKPLEQFVEKSSCAQPLGELTSLDAHGEFGGGSGSSPSSSSLCTEPLIPTTPIIPSEEMAKIACSLETKELWDKFHELGTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 T-box 20
别名 TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4
计算分子量 297 aa, 33 kDa
观测分子量50-60 kDa
GenBank蛋白编号BC120946
基因名称 TBX20
Gene ID (NCBI) 57057
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UMR3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.

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