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验证数据展示

  • WB analysis using 21429-1-AP (same clone as 21429-1-PBS)

    WB analysis using 21429-1-AP (same clone as 21429-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21429-1-AP (TBCCD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21429-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 21429-1-AP (same clone as 21429-1-PBS)

    IF Staining of HEK-293 using 21429-1-AP (same clone as 21429-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TBCCD1 antibody (21429-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21429-1-PBS in a different storage buffer formulation.

TBCCD1 Polyclonal antibody, PBS Only

TBCCD1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 21429-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

TBCC domain containing 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21429-1-PBS targets TBCCD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16032

Product name: Recombinant human TBCCD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 208-557 aa of BC025748

Sequence: AVVALSFLIEGTISRARKIYPLHELALWQPLHADSGFSKISKTFSFYKLETWLRSCLTGNPFGTSACLKSGKKLAWAHQVEGTTKRAKIACNTHVAPRMHRLVVMSQVYKQTLAKSSDTLAGAHVKIHRCNESFIYLLSPLRSVTIEKCRNSIFVLGPVGTTLHLHSCDNVKVIAVCHRLSISSTTGCIFHVLTPTRPLILSGNQTVTFAPFHTHYPMLEDHMARTGLATVPNYWDNPMVVCRENSDTRVFQLLPPCEFYVFIIPFEMEGDTTEIPGGLPSVYQKALGQREQKIQIWQKTVKEAHLTKDQRKQFQVLVENKFYEWLINTGHRQQLDSLVPPAAGSKQAAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBCC domain containing 1
别名 TBCC domain containing 1
计算分子量 557 aa, 64 kDa
观测分子量52 kDa
GenBank蛋白编号BC025748
基因名称 TBCCD1
Gene ID (NCBI) 55171
RRIDAB_3742033
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9NVR7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TBCC-domain containing 1 (TBCCD1) belongs to the tubulin-binding cofactor C (TBCC) family, which is a new human pericentriolar matrix component (PMID: 24101722). TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE-1 cells caused the dissociation of the centrosome from the nucleus and disorganization of the Golgi apparatus. TBCCD1 is a key regulator of centrosome positioning and consequently of internal cell organization (PMID: 20168327).

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