验证数据展示
经过测试的应用
| Positive IF-P detected in | human prostate cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67451 targets TAOK3 in IF-P applications and shows reactivity with Human samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag29146 Product name: Recombinant human TAOK3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 366-430 aa of BC002756 Sequence: SMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGDPRPEPRPTQSVQSQALHYR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | TAO kinase 3 |
| 别名 | DKFZp666H245, DPK, FLJ31808, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3 |
| 计算分子量 | 105 kDa |
| 观测分子量 | 100-105 kDa |
| GenBank蛋白编号 | BC002756 |
| 基因名称 | TAOK3 |
| Gene ID (NCBI) | 51347 |
| RRID | AB_2919477 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9H2K8 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TAOK3 antibody CL488-67451 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
