TAOK3 Monoclonal antibody
TAOK3 Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse and More (1)
Applications
IF-P, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1A5A9
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, A549 cells, LNCaP cells, K-562 cells |
| Positive IHC detected in | human prostate cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human prostate cancer tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67451-1-Ig targets TAOK3 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | IF-P, IHC, WB, ELISA |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Immunogen | TAOK3 fusion protein Ag29146 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | TAO kinase 3 |
| Synonyms | DKFZp666H245, DPK, FLJ31808, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3 |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 100-105 kDa |
| GenBank Accession Number | BC002756 |
| Gene Symbol | TAOK3 |
| Gene ID (NCBI) | 51347 |
| RRID | AB_2882685 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9H2K8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAOK3 antibody 67451-1-Ig | Download protocol |
| IHC protocol for TAOK3 antibody 67451-1-Ig | Download protocol |
| IF protocol for TAOK3 antibody 67451-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
