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Conjugate
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验证数据展示

  • FC experiment of HepG2 using CL488-67569

    FC experiment of HepG2 using CL488-67569

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TAF6L (CL488-67569, Clone:1G5C4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated TAF6L Monoclonal antibody

TAF6L Monoclonal Antibody for FC (Intra)
Cat No. CL488-67569

产品说明书

CloneNo. 1G5C4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

TAF6L, TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L, 1G5C4, PAF65 alpha, PAF65A, PAF65-alpha, PCAF-associated factor 65-alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67569-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67569 targets TAF6L in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag8202

Product name: Recombinant human TAF6L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC008785

Sequence: MSEREERRFVEIPRESVRLMAESTGLELSDEVAALLAEDVCYRLREATQNSSQFMKHTKRRKLTVEDFNRALRWSSVEAVCGYGSQEALPMRPAREGELYFPEDREVNLVELALATNIPKGCAETAVRVHVSYLDGKGNLAPQGSVPSAVSSLTDDLLKYYHQVTRAVLGDDPQLMKVALQDLQTNSKIGALLPYFVYVVSGVKSVSHDLEQLHRLLQVARSLFRNPHLCLGPYVRCLVGSVLYCVLEPLAASINPLNDHWTLRDGAALLLSHIFWTHGDLVSGLYQHILLSLQKILADPVRPLCCHYGAVVGLHALGWKAVERVLYPHLSTYWTNLQAVLDDYSVSNAQV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
别名 TAF6L, TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L, 1G5C4, PAF65 alpha, PAF65A, PAF65-alpha, PCAF-associated factor 65-alpha
计算分子量 622 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC008785
基因名称 TAF6L
Gene ID (NCBI) 10629
RRIDAB_2923777
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6J9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TAF6L antibody CL488-67569Download protocol
Standard Protocols
Click here to view our Standard Protocols