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TAF6L Monoclonal antibody

TAF6L Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G5C4

Cat No : 67569-1-Ig

Print datasheet

Synonyms

FLJ11136, PAF65 alpha, PAF65A, TAF6L

验证数据展示

  • WB analysis using 67569-1-Ig

    WB analysis using 67569-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67569-1-Ig (TAF6L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67569-1-Ig

    IHC staining of human breast cancer using 67569-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67569-1-Ig (TAF6L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67569-1-Ig targets TAF6L in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen TAF6L fusion protein Ag8202 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
Synonyms FLJ11136, PAF65 alpha, PAF65A, TAF6L
Calculated Molecular Weight 622 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC008785
Gene Symbol TAF6L
Gene ID (NCBI) 10629
RRIDAB_2882783
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6J9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF6L antibody 67569-1-IgDownload protocol
IHC protocol for TAF6L antibody 67569-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols