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验证数据展示

  • WB analysis using 67569-1-Ig

    WB analysis using 67569-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67569-1-Ig (TAF6L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67569-1-Ig

    IHC staining of human breast cancer using 67569-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67569-1-Ig (TAF6L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TAF6L Monoclonal antibody

TAF6L Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67569-1-Ig

产品说明书

CloneNo. 1G5C4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

1G5C4, PAF65 alpha, PAF65A, PAF65-alpha, PCAF-associated factor 65-alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67569-1-Ig targets TAF6L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8202

Product name: Recombinant human TAF6L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC008785

Sequence: MSEREERRFVEIPRESVRLMAESTGLELSDEVAALLAEDVCYRLREATQNSSQFMKHTKRRKLTVEDFNRALRWSSVEAVCGYGSQEALPMRPAREGELYFPEDREVNLVELALATNIPKGCAETAVRVHVSYLDGKGNLAPQGSVPSAVSSLTDDLLKYYHQVTRAVLGDDPQLMKVALQDLQTNSKIGALLPYFVYVVSGVKSVSHDLEQLHRLLQVARSLFRNPHLCLGPYVRCLVGSVLYCVLEPLAASINPLNDHWTLRDGAALLLSHIFWTHGDLVSGLYQHILLSLQKILADPVRPLCCHYGAVVGLHALGWKAVERVLYPHLSTYWTNLQAVLDDYSVSNAQV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
别名 1G5C4, PAF65 alpha, PAF65A, PAF65-alpha, PCAF-associated factor 65-alpha
计算分子量 622 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC008785
基因名称 TAF6L
Gene ID (NCBI) 10629
RRIDAB_2882783
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6J9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF6L antibody 67569-1-IgDownload protocol
IHC protocol for TAF6L antibody 67569-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols