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验证数据展示

  • WB analysis using 12818-1-AP (same clone as 12818-1-PBS)

    WB analysis using 12818-1-AP (same clone as 12818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12818-1-AP (TAF1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12818-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 12818-1-AP (same clone as 12818-1-PBS)

    IHC staining of human testis using 12818-1-AP (same clone as 12818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12818-1-AP (TAF1B antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12818-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 12818-1-AP (same clone as 12818-1-PBS)

    IHC staining of human testis using 12818-1-AP (same clone as 12818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12818-1-AP (TAF1B antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12818-1-PBS in a different storage buffer formulation.

TAF1B Polyclonal antibody, PBS Only

TAF1B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12818-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

RAF1B, RAFI63, RNA polymerase I-specific TBP-associated factor 63 kDa, SL1, TAFI63

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12818-1-PBS targets TAF1B in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3267

Product name: Recombinant human TAF1B protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-346 aa of BC018137

Sequence: MDLEESEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTIEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
别名 RAF1B, RAFI63, RNA polymerase I-specific TBP-associated factor 63 kDa, SL1, TAFI63
计算分子量 588 aa, 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC018137
基因名称 TAF1B
Gene ID (NCBI) 9014
RRIDAB_10644160
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53T94
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TAF1B is a component of the RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. It binds to rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex, and possibly directly through its interaction with RRN3 (PMID: 11250903; 11283244; 15970593; 21921199).

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