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TAF1B Polyclonal antibody

TAF1B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12818-1-AP

Print datasheet

Synonyms

RAF1B, RAFI63, SL1, TAF1B, TAFI63, TBP associated factor 1B

验证数据展示

  • WB analysis of HeLa using 12818-1-AP

    WB analysis of HeLa using 12818-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12818-1-AP (TAF1B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 12818-1-AP

    IHC staining of human testis using 12818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12818-1-AP (TAF1B antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 12818-1-AP

    IHC staining of human testis using 12818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12818-1-AP (TAF1B antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 12818-1-AP

    WB analysis using 12818-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12818-1-AP (TAF1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12818-1-AP targets TAF1B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen TAF1B fusion protein Ag3267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
Synonyms RAF1B, RAFI63, SL1, TAF1B, TAFI63, TBP associated factor 1B
Calculated Molecular Weight 588 aa, 69 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC018137
Gene Symbol TAF1B
Gene ID (NCBI) 9014
RRIDAB_10644160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53T94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF1B antibody 12818-1-APDownload protocol
IHC protocol for TAF1B antibody 12818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Commun

Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity.

Authors - Yanqin Yu
View Article