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验证数据展示

  • WB analysis of HaCaT using 68141-1-Ig

    WB analysis of HaCaT using 68141-1-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 68141-1-Ig (TACSTD2/TROP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 68141-1-Ig

    IHC staining of human breast cancer using 68141-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68141-1-Ig (TACSTD2/TROP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included
  • IHC staining of human breast cancer using 68141-1-Ig

    IHC staining of human breast cancer using 68141-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68141-1-Ig (TACSTD2/TROP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included
  • IF Staining of HaCaT using 68141-1-Ig

    IF Staining of HaCaT using 68141-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (68141-1-Ig, Clone: 5A6B1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human cervical cancer using 68141-1-Ig

    IHC staining of human cervical cancer using 68141-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68141-1-Ig (TACSTD2 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included
  • IF Staining of mouse skin using 68141-1-Ig

    IF Staining of mouse skin using 68141-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using TACSTD2/TROP2 antibody (68141-1-Ig, Clone: 5A6B1 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TACSTD2/TROP2 Monoclonal antibody

TACSTD2/TROP2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 68141-1-Ig

产品说明书

CloneNo. 5A6B1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

TACSTD2, 5A6B1, Cell surface glycoprotein Trop-2, EGP 1, GA733

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHaCaT cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skin tissue
Positive IF/ICC detected inHaCaT cells
Planning an IHC experiment? We recommend our IHCeasy TACSTD2 Ready-To-Use IHC Kit. TACSTD2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68141-1-Ig targets TACSTD2/TROP2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25775

Product name: Recombinant human TACSTD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 138-215 aa of BC009409

Sequence: KGDLSLRCDELVRTHHILIDLRHRPTAGAFNHSDLDAELRRLFRERYRLHPKFVAAVHYEQPTIQIELRQNTSQKAAG

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor-associated calcium signal transducer 2
别名 TACSTD2, 5A6B1, Cell surface glycoprotein Trop-2, EGP 1, GA733
计算分子量 36 kDa
观测分子量40-50 kDa
GenBank蛋白编号BC009409
基因名称 TROP2
Gene ID (NCBI) 4070
RRIDAB_3085043
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09758
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).

实验方案

Product Specific Protocols
IF protocol for TACSTD2/TROP2 antibody 68141-1-IgDownload protocol
IHC protocol for TACSTD2/TROP2 antibody 68141-1-IgDownload protocol
WB protocol for TACSTD2/TROP2 antibody 68141-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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