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验证数据展示

  • IF Staining of PC-12 using CL647-84627-5

    IF Staining of PC-12 using CL647-84627-5

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CoraLite® Plus 647 Syntaxin 6 antibody (CL647-84627-5, Clone: 242100A9 ) at dilution of 1:200.

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CoraLite® Plus 647-conjugated Syntaxin 6 Recombinant monoclonal antibody

Syntaxin 6 Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL647-84627-5

产品说明书

CloneNo. 242100A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

242100A9, STX6, Syntaxin-6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive IF/ICC detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 84627-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-84627-5 targets Syntaxin 6 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1284

Product name: Recombinant human STX6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-255 aa of BC009944

Sequence: MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 syntaxin 6
别名 242100A9, STX6, Syntaxin-6
计算分子量 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC009944
基因名称 Syntaxin 6
Gene ID (NCBI) 10228
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43752
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Syntaxin 6 antibody CL647-84627-5Download protocol
Standard Protocols
Click here to view our Standard Protocols