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验证数据展示

  • WB analysis using 84627-5-RR

    WB analysis using 84627-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84627-5-RR

    WB analysis using 84627-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 84627-5-RR

    IP experiment of mouse brain using 84627-5-RR

    IP result of anti-Syntaxin 6 (IP:84627-5-RR, 4ug; Detection:84627-5-RR 1:1500) with mouse brain tissue lysate 1840 ug.

  • IHC staining of mouse brain using 84627-5-RR

    IHC staining of mouse brain using 84627-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-12 using 84627-5-RR

    IF Staining of PC-12 using 84627-5-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (84627-5-RR, Clone: 242100A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84627-5-RR

    Affinity and Kinetic Characterization of 84627-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84627-5-RR against Human Syntaxin 6 were performed. The affinity constant is 1.55 nM.

    {{ptg:ProductFeaturesInfo}}

Syntaxin 6 Recombinant monoclonal antibody

Syntaxin 6 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 84627-5-RR

产品说明书

CloneNo. 242100A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Syntaxin-6, STX6, 242100A9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, PC-12 cells, Neuro-2a cells, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84627-5-RR targets Syntaxin 6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1284

Product name: Recombinant human STX6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-255 aa of BC009944

Sequence: MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 syntaxin 6
别名 Syntaxin-6, STX6, 242100A9
计算分子量 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC009944
基因名称 Syntaxin 6
Gene ID (NCBI) 10228
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO43752
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

实验方案

Product Specific Protocols
WB protocol for Syntaxin 6 antibody 84627-5-RRDownload protocol
IHC protocol for Syntaxin 6 antibody 84627-5-RRDownload protocol
IF protocol for Syntaxin 6 antibody 84627-5-RRDownload protocol
IP protocol for Syntaxin 6 antibody 84627-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols