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验证数据展示

  • FC experiment of PC-12 using CL488-10841

    FC experiment of PC-12 using CL488-10841

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Syntaxin 6 (CL488-10841) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of PC-12 using CL488-10841

    IF Staining of PC-12 using CL488-10841

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CoraLite® Plus 488 Syntaxin 6 antibody (CL488-10841) at dilution of 1:200, CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Syntaxin 6 Polyclonal antibody

Syntaxin 6 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10841

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Syntaxin-6, STX6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-12 cells
Positive FC (Intra) detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 10841-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10841 targets Syntaxin 6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Syntaxin 6 fusion protein Ag1284 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syntaxin 6
别名 Syntaxin-6, STX6
计算分子量 29 kDa
观测分子量 32 kDa
GenBank蛋白编号BC009944
基因名称 Syntaxin 6
Gene ID (NCBI) 10228
RRIDAB_2919003
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43752
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Syntaxin 6 antibody CL488-10841Download protocol
FC protocol for CL Plus 488 Syntaxin 6 antibody CL488-10841Download protocol
Standard Protocols
Click here to view our Standard Protocols