验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, human brain tissue, mouse brain tissue, rat brain tissue, A549 cells, PC-12 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-12 cells, murine BM-DC, murine Spleen-DC |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:350-1:1400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10841-1-AP targets Syntaxin 6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Syntaxin 6 fusion protein Ag1284 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin 6 |
| 别名 | Syntaxin-6, STX6 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC009944 |
| 基因名称 | Syntaxin 6 |
| Gene ID (NCBI) | 10228 |
| RRID | AB_2196506 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43752 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
| IHC protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
| IF protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
| IP protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science IRAP identifies an endosomal compartment required for MHC class I cross-presentation. | ||
Cell Rep Med Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis. | ||
Nat Commun IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses. | ||
Cell Rep Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation. |
