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验证数据展示

  • WB analysis using 85985-1-RR (same clone as 85985-1-PBS)

    WB analysis using 85985-1-RR (same clone as 85985-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85985-1-RR (Sftpd antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 85985-1-RR (same clone as 85985-1-PBS)

    IP experiment of mouse lung using 85985-1-RR (same clone as 85985-1-PBS)

    IP result of anti-Surfactant protein D (IP:85985-1-RR, 4ug; Detection:85985-1-RR 1:3000) with mouse lung tissue lysate 1760 ug. This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

  • IHC staining of rat lung using 85985-1-RR (same clone as 85985-1-PBS)

    IHC staining of rat lung using 85985-1-RR (same clone as 85985-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 85985-1-RR (same clone as 85985-1-PBS)

    IHC staining of mouse lung using 85985-1-RR (same clone as 85985-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02322-1

    Sandwich ELISA standard curve of MP02322-1

    Sandwich ELISA standard curve of MP02322-1, Mouse Surfactant protein D Recombinant Matched Antibody Pair - PBS only. 85985-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2924. 85985-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP02322-1

    Sample test of MP02322-1

    The mean Surfactant protein D concentration was determined to be 47.1 ng/mL in mouse lung tissue extract based on a 5.3 mg/mL extract load.

  • Sample test of MP02322-1

    Sample test of MP02322-1

    Mouse lung, cut into 1-2 mm pieces, was cultured unstimulated or stimulated with 1 μg/mL LPS for 3 days. The mean Surfactant protein D concentration was undetectable in unstimulated supernatant, 7.4 ng/mL in LPS stimulated supernatant.

  • Sample test of MP02322-1

    Sample test of MP02322-1

    Serum of sixteen mice was measured. The Surfactant protein D concentration of detected samples was determined to be 293.4 pg/mL with a range of 164.4-696.5 pg/mL.

Mouse Surfactant protein D Recombinant monoclonal antibody, PBS Only (Capture)

Surfactant protein D Uni-rAb® Recombinant Antibody for WB, IHC, IP, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85985-1-PBS

产品说明书

CloneNo. 250571G8

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IP, Sandwich ELISA, Indirect ELISA, Sample test

别名

Lung surfactant protein D, PSP-D, Pulmonary surfactant-associated protein D, Sftp4, Sftpd

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85985-1-PBS targets Surfactant protein D as part of a matched antibody pair:

MP02322-1: 85985-1-PBS capture and 85985-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IP, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2924

Product name: Recombinant Mouse Surfactant protein D protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-374 aa of NM_009160.2

Sequence: AEMKSLSQRSVPNTCTLVMCSPTENGLPGRDGRDGREGPRGEKGDPGLPGPMGLSGLQGPTGPVGPKGENGSAGEPGPKGERGLSGPPGLPGIPGPAGKEGPSGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSTGAKGSTGPKGERGAPGVQGAPGNAGAAGPAGPAGPQGAPGSRGPPGLKGDRGVPGDRGIKGESGLPDSAALRQQMEALKGKLQRLEVAFSHYQKAALFPDGRSVGDKIFRTADSEKPFEDAQEMCKQAGGQLASPRSATENAAIQQLITAHNKAAFLSMTDVGTEGKFTYPTGEPLVYSNWAPGEPNNNGGAENCVEIFTNGQWNDKACGEQRLVICEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 surfactant associated protein D
别名 Lung surfactant protein D, PSP-D, Pulmonary surfactant-associated protein D, Sftp4, Sftpd
计算分子量38 kDa
观测分子量40 kDa
GenBank蛋白编号NM_009160.2
基因名称 Sftpd
Gene ID (NCBI) 20390
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP50404
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Surfactant protein D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.

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