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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Sortilin Polyclonal antibody

Sortilin Polyclonal Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-12369

Print datasheet

Synonyms

100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3, SORT1, Sortilin, sortilin 1

验证数据展示

  • IF Staining of mouse brain using CL647-12369

    IF Staining of mouse brain using CL647-12369

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

  • IF Staining of mouse brain using CL647-12369

    IF Staining of mouse brain using CL647-12369

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

  • FC experiment of Neuro-2a using CL647-12369

    FC experiment of Neuro-2a using CL647-12369

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Sortilin (CL647-12369) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inmouse brain tissue
Positive FC detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy SORT1 Ready-To-Use IHC Kit. SORT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12369-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-12369 targets Sortilin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity human, mouse, rat
Immunogen Sortilin fusion protein Ag2997 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sortilin 1
Synonyms 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3, SORT1, Sortilin, sortilin 1
Calculated Molecular Weight 831 aa, 92 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC023542
Gene Symbol SORT1
Gene ID (NCBI) 6272
ENSEMBL Gene IDENSG00000134243
RRIDAB_2920221
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99523
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Sortilin antibody CL647-12369Download protocol
FC protocol for CL Plus 647 Sortilin antibody CL647-12369Download protocol
Standard Protocols
Click here to view our Standard Protocols