• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of mouse brain using CL647-12369

    IF Staining of mouse brain using CL647-12369

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

  • IF Staining of mouse brain using CL647-12369

    IF Staining of mouse brain using CL647-12369

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

  • FC experiment of Neuro-2a using CL647-12369

    FC experiment of Neuro-2a using CL647-12369

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Sortilin (CL647-12369) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated Sortilin Polyclonal antibody

Sortilin Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL647-12369

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy SORT1 Ready-To-Use IHC Kit. SORT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12369-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-12369 targets Sortilin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Sortilin fusion protein Ag2997 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sortilin 1
别名 SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95
计算分子量 831 aa, 92 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC023542
基因名称 Sortilin
Gene ID (NCBI) 6272
ENSEMBL Gene IDENSG00000134243
RRIDAB_2920221
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99523
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Sortilin antibody CL647-12369Download protocol
Standard Protocols
Click here to view our Standard Protocols