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验证数据展示

  • WB analysis using 12369-1-AP

    WB analysis using 12369-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12369-1-AP

    IHC staining of mouse brain using 12369-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SORT1 antibody included
  • IHC staining of mouse brain using 12369-1-AP

    IHC staining of mouse brain using 12369-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SORT1 antibody included
  • IF Staining of mouse brain using 12369-1-AP

    IF Staining of mouse brain using 12369-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 12369-1-AP

    IF Staining of mouse brain using 12369-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Neuro-2a using 12369-1-AP

    FC experiment of Neuro-2a using 12369-1-AP

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human Sortilin (12369-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IP experiment of mouse brain using 12369-1-AP

    IP experiment of mouse brain using 12369-1-AP

    IP result of anti-Sortilin (IP:12369-1-AP, 4ug; Detection:12369-1-AP 1:3000) with mouse brain tissue lysate 1400 ug.

  • IF Staining of HeLa using 12369-1-AP

    IF Staining of HeLa using 12369-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sortilin antibody (12369-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

Sortilin Polyclonal antibody

Sortilin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 12369-1-AP

产品说明书

引用文献(27)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

别名

SORT1, 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy SORT1 Ready-To-Use IHC Kit. SORT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12369-1-AP targets Sortilin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag2997

Product name: Recombinant human SORT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 481-831 aa of BC023542

Sequence: EPNAVGIVIAHGSVGDAISVMVPDVYISDDGGYSWTKMLEGPHYYTILDSGGIIVAIEHSSRPINVIKFSTDEGQCWQTYTFTRDPIYFTGLASEPGARSMNISIWGFTESFLTSQWVSYTIDFKDILERNCEEKDYTIWLAHSTDPEDYEDGCILGYKEQFLRLRKSSVCQNGRDYVVTKQPSICLCSLEDFLCDFGYYRPENDSKCVEQPELKGHDLEFCLYGREEHLTTNGYRKIPGDKCQGGVNPVREVKDLKKKCTSNFLSPEKQNSKSNSVPIILAIVGLMLVTVVAGVLIVKKYVCGGRFLVHRYSVLQQHAEANGVDGVDALDTASHTNKSGYHDDSDEDLLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sortilin 1
别名 SORT1, 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3
计算分子量 831 aa, 92 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC023542
基因名称 Sortilin
Gene ID (NCBI) 6272
ENSEMBL Gene IDENSG00000134243
RRIDAB_2302242
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99523
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

实验方案

Product Specific Protocols
FC protocol for Sortilin antibody 12369-1-APDownload protocol
IF protocol for Sortilin antibody 12369-1-APDownload protocol
IHC protocol for Sortilin antibody 12369-1-APDownload protocol
IP protocol for Sortilin antibody 12369-1-APDownload protocol
WB protocol for Sortilin antibody 12369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

EHBP1 suppresses liver fibrosis in metabolic dysfunction-associated steatohepatitis

Authors - Fanglin Ma
View Article
humanIF

Autophagy

Impaired chaperone-mediated autophagy leads to abnormal SORT1 (sortilin 1) turnover and CES1-dependent triglyceride hydrolysis

Authors - You-Jin Choi
View Article
mouseWB,IP,IF

Nat Commun

Sortilin-mediated translocation of mitochondrial ACSL1 impairs adipocyte thermogenesis and energy expenditure in male mice

Authors - Min Yang
  • KO Validated
View Article
mouse,humanWB,IP,IF

Theranostics

RPS23RG1 inhibits SORT1-mediated lysosomal degradation of MDGA2 to protect against autism

Authors - Yuanhui Huo
View Article
humanWB,IF

Adv Sci (Weinh)

Dysfunctional Decidual CD4+T Cells Induce Recurrent Pregnancy Loss via Palmitoylation-Dependent Tim-3 Lysosomal Sorting and Degradation

Authors - Liyuan Cui
View Article
humanWB

EBioMedicine

Neurotensin contributes to pediatric intestinal failure-associated liver disease via regulating intestinal bile acids uptake.

Authors - Yongtao Xiao
  • KD Validated
View Article
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