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验证数据展示

  • WB analysis using 12369-1-AP

    WB analysis using 12369-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12369-1-AP

    IHC staining of mouse brain using 12369-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SORT1 antibody included
  • IHC staining of mouse brain using 12369-1-AP

    IHC staining of mouse brain using 12369-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SORT1 antibody included
  • IF Staining of mouse brain using 12369-1-AP

    IF Staining of mouse brain using 12369-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 12369-1-AP

    IF Staining of mouse brain using 12369-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Neuro-2a using 12369-1-AP

    FC experiment of Neuro-2a using 12369-1-AP

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human Sortilin (12369-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis of SKOV-3 using 12369-1-AP

    WB analysis of SKOV-3 using 12369-1-AP

    WB result of Sortilin antibody (12369-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Sortilin transfected SKOV-3 cells.

  • IP experiment of mouse brain using 12369-1-AP

    IP experiment of mouse brain using 12369-1-AP

    IP result of anti-Sortilin (IP:12369-1-AP, 4ug; Detection:12369-1-AP 1:3000) with mouse brain tissue lysate 1400 ug.

  • IF Staining of HeLa using 12369-1-AP

    IF Staining of HeLa using 12369-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sortilin antibody (12369-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

Sortilin Polyclonal antibody

Sortilin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 12369-1-AP

产品说明书

引用文献(29)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

别名

SORT1, 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, SKOV-3 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy SORT1 Ready-To-Use IHC Kit. SORT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12369-1-AP targets Sortilin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag2997

Product name: Recombinant human SORT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 481-831 aa of BC023542

Sequence: EPNAVGIVIAHGSVGDAISVMVPDVYISDDGGYSWTKMLEGPHYYTILDSGGIIVAIEHSSRPINVIKFSTDEGQCWQTYTFTRDPIYFTGLASEPGARSMNISIWGFTESFLTSQWVSYTIDFKDILERNCEEKDYTIWLAHSTDPEDYEDGCILGYKEQFLRLRKSSVCQNGRDYVVTKQPSICLCSLEDFLCDFGYYRPENDSKCVEQPELKGHDLEFCLYGREEHLTTNGYRKIPGDKCQGGVNPVREVKDLKKKCTSNFLSPEKQNSKSNSVPIILAIVGLMLVTVVAGVLIVKKYVCGGRFLVHRYSVLQQHAEANGVDGVDALDTASHTNKSGYHDDSDEDLLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sortilin 1
别名 SORT1, 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3
计算分子量 831 aa, 92 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC023542
基因名称 Sortilin
Gene ID (NCBI) 6272
ENSEMBL Gene IDENSG00000134243
RRIDAB_2302242
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99523
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

实验方案

Product Specific Protocols
FC protocol for Sortilin antibody 12369-1-APDownload protocol
IF protocol for Sortilin antibody 12369-1-APDownload protocol
IHC protocol for Sortilin antibody 12369-1-APDownload protocol
IP protocol for Sortilin antibody 12369-1-APDownload protocol
WB protocol for Sortilin antibody 12369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

EHBP1 suppresses liver fibrosis in metabolic dysfunction-associated steatohepatitis

Authors - Fanglin Ma
View Article
humanIF

Autophagy

Impaired chaperone-mediated autophagy leads to abnormal SORT1 (sortilin 1) turnover and CES1-dependent triglyceride hydrolysis

Authors - You-Jin Choi
View Article
mouseWB,IP,IF

Nat Commun

Sortilin-mediated translocation of mitochondrial ACSL1 impairs adipocyte thermogenesis and energy expenditure in male mice

Authors - Min Yang
  • KO Validated
View Article
mouse,humanWB,IP,IF

Theranostics

RPS23RG1 inhibits SORT1-mediated lysosomal degradation of MDGA2 to protect against autism

Authors - Yuanhui Huo
View Article
humanWB,IF

Adv Sci (Weinh)

Dysfunctional Decidual CD4+T Cells Induce Recurrent Pregnancy Loss via Palmitoylation-Dependent Tim-3 Lysosomal Sorting and Degradation

Authors - Liyuan Cui
View Article
humanWB

EBioMedicine

Neurotensin contributes to pediatric intestinal failure-associated liver disease via regulating intestinal bile acids uptake.

Authors - Yongtao Xiao
  • KD Validated
View Article
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