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验证数据展示

  • IF Staining of MDCK using CL488-66542

    IF Staining of MDCK using CL488-66542

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 Septin 7 antibody (CL488-66542, Clone: 1G12D1 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Septin 7 Monoclonal antibody

Septin 7 Monoclonal Antibody for IF/ICC
Cat No. CL488-66542

产品说明书

CloneNo. 1G12D1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

septin7, SEPT7, SEPT 7, CDC3, CDC10 protein homolog

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy SEPT7 Ready-To-Use IHC Kit. SEPT7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66542-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66542 targets Septin 7 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4309

Product name: Recombinant human SEPT7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 110-417 aa of BC025987

Sequence: VIDYIDSKFEDYLNAESRVNRRQMPDNRVQCCLYFIAPSGHGLKPLDIEFMKRLHEKVNIIPLIAKADTLTPEECQQFKKQIMKEIQEHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENGEHCDFTILRNMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNKNKGQLTKSPLAQMEEERREHVAKMKKMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRQFEDEKANWEAQQRILEQQNSSRTLEKNKKKGKIF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 septin 7
别名 septin7, SEPT7, SEPT 7, CDC3, CDC10 protein homolog
计算分子量 51 kDa
观测分子量 48-51 kDa
GenBank蛋白编号BC025987
基因名称 Septin 7
Gene ID (NCBI) 989
RRIDAB_3672913
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ16181
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. Septin 7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Septin 7 antibody CL488-66542Download protocol
Standard Protocols
Click here to view our Standard Protocols
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