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Septin 7 Monoclonal antibody

Septin 7 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G12D1

Cat No : 66542-1-Ig

Print datasheet

Synonyms

CDC10, CDC10 protein homolog, CDC3, Nbla02942, SEPT7, SEPT7A, septin 7, septin7

验证数据展示

  • WB analysis using 66542-1-Ig

    WB analysis using 66542-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66542-1-Ig (Septin 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 66542-1-Ig

    IHC staining of human gliomas using 66542-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 66542-1-Ig

    IHC staining of human gliomas using 66542-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 66542-1-Ig

    WB analysis of HepG2 using 66542-1-Ig

    WB result of Septin 7 antibody (66542-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 7 transfected HepG2 cells.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 tissue
Positive IHC detected inhuman gliomas tissue, human lung tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66542-1-Ig targets Septin 7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen Septin 7 fusion protein Ag4309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name septin 7
Synonyms CDC10, CDC10 protein homolog, CDC3, Nbla02942, SEPT7, SEPT7A, septin 7, septin7
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 48-51 kDa
GenBank Accession NumberBC025987
Gene Symbol SEPT7
Gene ID (NCBI) 989
RRIDAB_2881904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16181
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. Septin 7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

实验方案

Product Specific Protocols
WB protocol for Septin 7 antibody 66542-1-IgDownload protocol
IHC protocol for Septin 7 antibody 66542-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell Int

The clustering status of detached gastric cancer cells inhibits anoikis-induced ferroptosis to promote metastatic colonization

Authors - Juan Sun
View Article