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验证数据展示

  • WB analysis using 66542-1-Ig (same clone as 66542-1-PBS)

    WB analysis using 66542-1-Ig (same clone as 66542-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66542-1-Ig (Septin 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66542-1-Ig (same clone as 66542-1-PBS)

    IHC staining of human gliomas using 66542-1-Ig (same clone as 66542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEPT7 antibody included
  • IHC staining of human gliomas using 66542-1-Ig (same clone as 66542-1-PBS)

    IHC staining of human gliomas using 66542-1-Ig (same clone as 66542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEPT7 antibody included
  • WB analysis of HepG2 using 66542-1-Ig (same clone as 66542-1-PBS)

    WB analysis of HepG2 using 66542-1-Ig (same clone as 66542-1-PBS)

    WB result of Septin 7 antibody (66542-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 7 transfected HepG2 cells. This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Septin 7 Monoclonal antibody, PBS Only

Septin 7 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66542-1-PBS

产品说明书

CloneNo. 1G12D1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

septin7, 1G12D1, CDC10, CDC10 protein homolog, CDC3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SEPT7 Ready-To-Use IHC Kit. SEPT7 primary antibody included.

产品信息

66542-1-PBS targets Septin 7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4309

Product name: Recombinant human SEPT7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 110-417 aa of BC025987

Sequence: VIDYIDSKFEDYLNAESRVNRRQMPDNRVQCCLYFIAPSGHGLKPLDIEFMKRLHEKVNIIPLIAKADTLTPEECQQFKKQIMKEIQEHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENGEHCDFTILRNMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNKNKGQLTKSPLAQMEEERREHVAKMKKMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRQFEDEKANWEAQQRILEQQNSSRTLEKNKKKGKIF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 septin 7
别名 septin7, 1G12D1, CDC10, CDC10 protein homolog, CDC3
计算分子量 51 kDa
观测分子量 48-51 kDa
GenBank蛋白编号BC025987
基因名称 Septin 7
Gene ID (NCBI) 989
RRIDAB_2881904
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ16181
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. Septin 7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

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