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验证数据展示

  • WB analysis of SK-N-SH using 85108-5-RR (same clone as 85108-5-PBS)

    WB analysis of SK-N-SH using 85108-5-RR (same clone as 85108-5-PBS)

    SK-N-SH cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 85108-5-RR (same clone as 85108-5-PBS)

    WB analysis of U-251 using 85108-5-RR (same clone as 85108-5-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85108-5-RR

    Affinity and Kinetic Characterization of 85108-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85108-5-RR against Human SYTL5 were performed. The affinity constant is below 1 pM.

SYTL5 Recombinant antibody, PBS Only

SYTL5 Uni-rAbTM Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 85108-5-PBS

产品说明书

CloneNo. 242838D12

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF-P, Indirect ELISA

别名

Synaptotagmin-like protein 5, Synaptotagmin like protein 5, synaptotagmin like 5, SLP5, 242838D12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85108-5-PBS targets SYTL5 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 SYTL5 fusion protein Ag21698 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptotagmin-like 5
别名 Synaptotagmin-like protein 5, Synaptotagmin like protein 5, synaptotagmin like 5, SLP5, 242838D12
计算分子量 730 aa, 81 kDa
观测分子量82 kDa
GenBank蛋白编号BC131585
基因名称 SYTL5
Gene ID (NCBI) 94122
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDW5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SYTL5, also known as SLP5, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by the presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL5 promotes the development and progression of papillary thyroid carcinoma (PTC) by activating the NF-κB signaling pathway (PMID: 36378561).