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验证数据展示

  • WB analysis of SK-N-SH using 85108-5-RR (same clone as 85108-5-PBS)

    WB analysis of SK-N-SH using 85108-5-RR (same clone as 85108-5-PBS)

    SK-N-SH cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 85108-5-RR (same clone as 85108-5-PBS)

    WB analysis of U-251 using 85108-5-RR (same clone as 85108-5-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    IHC staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85108-5-RR (SYTL5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85108-5-RR

    Affinity and Kinetic Characterization of 85108-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85108-5-RR against Human SYTL5 were performed. The affinity constant is below 1 pM.

SYTL5 Recombinant monoclonal antibody, PBS Only

SYTL5 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85108-5-PBS

产品说明书

CloneNo. 242838D12

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

242838D12, SLP5, synaptotagmin like 5, Synaptotagmin like protein 5, Synaptotagmin-like protein 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85108-5-PBS targets SYTL5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag21698

Product name: Recombinant human SYTL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 56-343 aa of BC131585

Sequence: TQQEASRVCVHCHRNLGLIFDRGDPCQACSLRVCRECRVAGPNGSWKCTVCDKIAQLRIITGEWFFEEKAKRFKQVNVLGTDVVRQSILRRSPGAEEVQSQEQTRQDAEKSDTSPVAGKKASHDGPKRKGFLLSKFRSATRGEIITPKTDTGRSYSLDLDGQHFRSLKSPPGSDRGSTGSSDLNDQEPGPRTPKSSRSNGVTPGTQSSPAPSTRTVTSVISREYGFENSMDLAAIEGTSQELTKSHRRNTSGTPSIAVSGTSLSSDQSRSELDLSESFTEDSEDTVSI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptotagmin-like 5
别名 242838D12, SLP5, synaptotagmin like 5, Synaptotagmin like protein 5, Synaptotagmin-like protein 5
计算分子量 730 aa, 81 kDa
观测分子量82 kDa
GenBank蛋白编号BC131585
基因名称 SYTL5
Gene ID (NCBI) 94122
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDW5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SYTL5, also known as SLP5, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by the presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL5 promotes the development and progression of papillary thyroid carcinoma (PTC) by activating the NF-κB signaling pathway (PMID: 36378561).

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